Composite

Part:BBa_K3753015:Design

Designed by: Yiqing Zhang   Group: iGEM21_NJTech_China   (2021-10-16)


UASCLB-pTEF-GFP-CYC1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 747
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 747
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 747
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 747
    Illegal NgoMIV site found at 1269
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2423


Design Notes

The UASCLB and the promoter of tef2 was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The green fluorescent protein and the terminator CYC1(GFP-CYC1) was obtained by PCR amplification using pRS415-GFP-CYC1 plasmid as template.

Source

Saccharomyces cerevisiae

References